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KMID : 0620920080400010130
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Experimental & Molecular Medicine
2008 Volume.40 No. 1 p.130 ~ p.144
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Anti-tumor immunostimulatory effect of heat-killed tumor cells
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Yoon Taek-Joon
Lee Chang-Kwon
Kim Ji-Yeon
Kim Hyo-Jeong
Hong Chang-Wan
Lee Hyun-Ji
Lee Kwang-Ho
Hoog Seok-Mann
Park Se-Ho
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Abstract
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As a part of our ongoing search for a safe and efficient anti-tumor vaccine, we attempted to determine whether the molecular nature of certain tumor antigens would influence immune responses against tumor cells. As compared with freeze-thawed or formaldehyde- fixed tumor antigens, heat-denatured tumor antigens elicited profound anti-tumor immune responses and greatly inhibited the growth of live tumor cells. The heat-denatured tumor antigens induced a substantial increase in the anti-tumor CTL response in the absence of any adjuvant material. This response appears to be initiated by strong activation of the antigen- presenting cells, which may recognize heat-denatured protein antigens. Upon recognition of the heat-denatured tumor antigens, macrophages and dendritic cells were found to acutely upregulate the expression of co-stimulatory molecules such as B7.2, as well as the secretion of inflammatory cytokines such as IL-12 and TNF-α . The results of this study indicate that heat-denatured tumor extracts might elicit protective anti-tumor adaptive immune responses and also raise the possibility that a safe and efficient adjuvant- free tumor vaccine might be developed in conjunction with a dendritic cell-based tumor vaccine.
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KEYWORD
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cancer vaccines, dendritic cells, immunotherapy, active, interleukin 12, mice
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